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Microbiological monitoring of polyantibiotic resistant strains of Pseudomonas aeruginosa as one of the measures for the prevention of nosocomyal

Microbiological monitoring of polyantibiotic resistant strains of Pseudomonas aeruginosa as one of the measures for the prevention of nosocomyal

ISSN 2223-6775 Ukrainian journal of occupational health Vol.17, No 1, 2021

Microbiological monitoring of polyantibiotic resistant strains of Pseudomonas aeruginosa as one of the measures for the prevention of nosocomyal

Sarkis-Ivanova V.V., Dreval M.V., Korobchanski V.A.

https://doi.org/10.33573/ujoh2021.01.053

Kharkiv National Medical University, Kharkiv

Full article (PDF)

Introduction. Pseudomonas aeruginosa is a leading causative agent of nosocomial infections. Currently, the problem of prevention and treatment of Pseudomonas aeruginosa infection is a high level of protection of bacteria from the action of antibacterial agents. Microbiological monitoring of resistance profiles of clinical strains of Pseudomonas aeruginosa is appropriate for use in planning preventive measures for nosocomial infection.

Purpose and research. Determination of sensitivity of test strains of Pseudomonas aeruginosa to antibiotics, which were isolated from clinical material from patients of inpatient treatment and treatment facilities in Kharkov, as well as improvement of measures to prevent nosocomial infection.

Materials and methods of research. 90 cultures of Pseudomonas aeruginosa were used in the work. Test isolates of Pseudomonas aeruginosa were isolated and identified. The research used certified nutrient media produced by the Experimental Plant of Medicines, Ukraine, bioMérieux, France, HiMedia Laboratories Pvt. Limited, India. Determination of the sensitivity of the removed cultures of microorganisms to antimicrobial drugs was performed by the Bauer-Kirbi disco-diffusion method on Hinton-Müller medium using ready-made commercial disks, and analyzed using the computer program WHONET5.1.

Results. A significant difference in the levels of resistance of Pseudomonas aeruginosa test strains was found during antibiotic susceptibility analysis.

Conclusions. The focus of microbiological monitoring on the timely active detection of nosocomial infections fully reflects the epidemiological situation in the hospital. Sanitary and epidemiological measures carried out based on the results of microbiological monitoring will reduce the incidence and development of purulent-septic diseases in the hospital.

Key words: Pseudomonas aeruginosa, polyantibiotic resistance, nosocomial infection, preventive measures.

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